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In this paper, a description of sentential negation makers in Russian Sign Language (RSL) is provided. Two types of these markers exist. The first one is a negative sign – a manual negator. The second type is markers that are expressed by facial expressions, and head movements – non-manual negation. Moreover, this observation of sentential negation presents the frequency of manual negators, and the distribution of non-manual negation. The data also shows that in negative constructions the basic word order changes. In addition, I offer an analysis that deals with this phenomenon.